For some people there egg whites will not float on top of semen. We discovered the reason for this is the sugar content in the semen. If you don't have enough sugar then the density is off. You can fix this. Please remember, to much sugar will kill sperm so please try to stick to the suggested amount or less. Sugar water does not change pH.
Generally the rule is mix 1 tsp sugar with 2tsp distilled water and dissolve the sugar in the water. Then collect your semen, and add SW that is half the amount of semen to the sample.
So for example, if you had 2ml of semen, then you would add 1 ml of sw. Some people are adding just a little less of SW, say 0.9ml to see if that works. So now you have 3ml sample (2ml semen, 1ml sw) and you mix that by rocking back and forth. THEN you add EW on top that is the same amount as the semen you originally collected, in this eg, it is 2ml.
So then in your incubator you would have a 5ml sample, 3ml would be the sw/semen mix and the top 2ml would be EW.
Shorter version for reference:
SW = 1 tsp sugar to 2 tsp distilled water
Mix 1/2 semen volume of SW with Semen (rocking motion)
add EW same volume as original semen volume.
I've been using tablespoons to mix the SW, then I have a lot in case of a mistake. teaspoons are pretty small and harder to work with, but I think as long as the ratio is the same it should be OK right?