MSU Question TTC boy

Hi,

Just wondering....would it be better to put EW on top of the final sample in the Instead cup as well as putting some on your cervix???

 Also, we seem to have trouble with our EW sinking. We are now using smaller test tubes 11.5 X 76mm, with better results. I find that it is perfectly separated before we put it in the incubator, but once we pull it out, the sample is slightly mixed with cylcone patterns. When I look at it under the microscope, I can still see the Super X's in the first layer and the straight swimmers in the secondish layer, but the layer between the sperm and the EW is not clearly defined. Should I try using mesh with a bigger test tube???

Also, does anyone know if there any better photos of a successful sort other than the ones in the experiment photos???

 Would appreciate any advice...

 Thanks

Just wondering....would it be better to put EW on top of the final sample in the Instead cup as well as putting some on your cervix???

you can do that, but its not needed

 Also, we seem to have trouble with our EW sinking. We are now using smaller test tubes 11.5 X 76mm, with better results. I find that it is perfectly separated before we put it in the incubator, but once we pull it out, the sample is slightly mixed with cylcone patterns. When I look at it under the microscope, I can still see the Super X's in the first layer and the straight swimmers in the secondish layer, but the layer between the sperm and the EW is not clearly defined. Should I try using mesh with a bigger test tube???

bigger test tube your ew may sink. dont use the layer before it touches the seperm, only use ew

Also, does anyone know if there any better photos of a successful sort other than the ones in the experiment photos???

there is a thread with a bunch of pics

 Thanks, Tamara.

We tried again last night with SW, but the sperm still didn't float??? The sperm was still alive but it had mixed as we colored the EW this time also.

 If we use mesh, what mesh is recommended??

 Sorry....also should the sugar water be warmed to room temp too.

 Thanks.

Ok...I've got the mesh...some fine netting. Should I use the thick part of the EW or the runny bit???

Just found the answer in another area and will share.....

• Should you use the thinnest part of the EW or the thickest?