My MSU Refrigerator vs Incubator Experiment

February 8, 2010

MSU 1 hr Refrigerator vs Incubator

48 hr since last sample / release

 

                                    REFRIGERATOR                             INCUBATOR

-10 min 1:50p	4 mL sample on counter to liquefy
0 min	2.0 mL in shot glass in fridge @ 36*	2.0 mL in shot glass in incubator @ 99*
1 hr 1st 0.5 ml	20% content 10/90 x/y	40% content 30/70 x/y
1 hr 2nd 1 mL in the middle	30% content 10/90 x/y Although there don’t seem to be as many swimmers, they all seem to know exactly where they are going, very straight. Only a small few are circling	50% content 20/80 x/y A lot more swimmers moving around, there seems to be a few more moving slower, not as determined to go straight
1.5 hr 3rd  1 mL from very bottom	50% content 80/20 Definitely more sperm per area Most slow moving circles, still a few remaining straight shooters	70% content 70/30 Most seem confusing, going in circles but still some “determined” straight Ys are obvious
2 hr 2nd 1 mL in the middle	20% content 30/70 After 1 hr on the counter Still a few straight shooter but seems to be equal slow circlers	40% content 30/70 Still seems to be more straight shooters

 

Conclusions:

Disclaimer – I am not a scientist, I am a stay at home mom. I went to school for Architecture, so these experiments are just my thoughts.

1)      Straight out of the fridge, the second layer was full of Y’s (10/90), but when tested that layer again after 1 hr on the counter, a lot of the Y’s seemed to die off OR more X’s woke up, I don’t know which. There seemed to be less swimmers on the slide after the 1 hr on the counter, so I am asuuming more Y’s died off.  Perhaps due to temperature change so many times?

2)      Straight out of the incubator, the second layer had majority Y’s also. But there was also a lot more swimmers on the slide (content). So, although the incubator ratio for x/y was a little less Y’s than the refrigerator, there were more left alive, so the chances of getting pregnant would be higher, with a slightly less chance of it being a boy.

3)       With the remaining bottom 1 mL, there were more swimmers left alive in the incubator sample, with 70/30 x/y ratio vs the refrigerated sample of 80/20 x/ys, but the amount of moving swimmers was less with the refrigerated sample.  So you would have a higher chance of getting pregnant with the incubated sample, with a slightly less concentration of X swimmers.

the only thing that worries me is we have no studies to back up these claims.  And since you and I aren't scientist we can't back up our thoughts.. can't sight our sources.

If you want to keep these up, I'll start trying to look for any studies

 Oh I know.  I'm just doing these for my own interests, to find the best way for me to get my DD. And since I'm documenting them anyways, just thought I'd copy and paste into the forum.

PLEASE NOTE: I am a STAY AT HOME MOM.... these studies are just for 'fun'. These findings are not fact, just my observations. 

I don't want anyone putting their "money" on my findings.  If you think it makes sense, and would strong suggest experimenting for yourself. 

 Just curious and I still am very interested in thie rm but I was reading a book about infertility and it went into sperm count alot and basically said that the X and Y sperms you cant tell under a microscope they all swim equal but the one you see dead doing zig zags and circles are poorly formed sperm and have something wrong with the egm two head two tails ect. So thinking that the methos is just making all the sperm sick and swiming badly?

and if you stress them more sick and whan you add environment like ew lime they love it and swim beter!

I was hoping someone could change my mind so I have faith again, I so want to believe. I will still do ecg because it has study's saying low sperm count favours girls but not convinced on what degree I should do egs I am deff doing lime 0=12.

sick sperm can not fertilize an egg.  sperm with 2 tails or 2 heads can't do it either

If your talking about chromosomal defects, we can't sort these one way or another.. only microsort can see DNA. 

lets say a sperm has down syndrome in its DNA.  When we sort x and y sperm the x down syndrome sperm will still stay with the x sperm and the y down syndrome sperm will still swim up to the high pH ew with all the other y sperm.

We are trying to sort sperm with the idea that they are attracted to different things.  The sperm  bring along the DNA, good or bad

I think I am finally getting it. We don't have any specific facts saying x sperm swim in circles and y sperm swim in circles but we DO know that y sperm are, for a fact, attracted to higher ph egg proteins. Knowing this, when you see the sample in the EW, that it is majority of straight swimming spermies we can conjecture that that is how y sperm swim. I was a little lost with it but I think I finally got it! Is this what we base SU on Tamara?

Dwigt:

I think I am finally getting it. We don't have any specific facts saying x sperm swim in circles and y sperm swim in circles but we DO know that y sperm are, for a fact, attracted to higher ph egg proteins. Knowing this, when you see the sample in the EW, that it is majority of straight swimming spermies we can conjecture that that is how y sperm swim. I was a little lost with it but I think I finally got it! Is this what we base SU on Tamara?

yup you got it!

I guess I said it wrong I ment is it just you see more slower circle sperm when the environment is low ph and straight fast ones in high ph?

I mean if you put a straight swimming Y looking sperm in a low ph would it swim in circles because it dosn't like it? and vice versa?

Um mabey im just asking for proff Y like a more high ph I will go and check ou the studies in gold unless you know on any?

Thanks Tamara

I guess if it was that way you wouldn't see both sperm types in low and high ph?

Ariel:

im just asking for proff Y like a more high ph

http://www.in-gender.com/cs/forums/t/71062.aspx

Also the gold studies for msu go into pH and sperm

pH Studies: 

 

"CM pH from 9-9.9 favors boys"

Y sperm thrive in a high cm pH, however you don't want it to be any higher than 9.9 for cm and ew (dh can be higher).  You can change cm pH threw diet, supplements,  baking soda douche) or with lubricant (pre-seed. but it isnt' high enough for a great sway) 

1975: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&list_uids=1065697&dopt=Citation

 

1981:  This study shows that pH can change the ion composition of the sperm- high pH favoring boy sperm, low pH favoring girl sperm.  It also goes into a theory about egg polarity.    

http://www.freepatentsonline.com/4276139.html

1983:  This study shows that a high pH not only favors boys but it causes sperm to swim faster.  This studies has a bunch of fun graphs and charts to:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=393590

 

1987 http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3675523&dopt=Citation

1988:  Chemical balance of the female genital tract have an effect on sex ratio at conception

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=3071849&dopt=Citation

2004:  pH has a impact in sex selection along with several other theories.  He goes on to say that in most humans pH is Highest at time of O: 

http://humrep.oxfordjournals.org/cgi/content/full/19/6/1250

 

2005:  Claims that it proves that high pH favors boys.  It also goes on to say that moon can have a effect on cm pH.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16295726&dopt=Citation

    

2006:  This study shows that different types of CM can select different types of sperm:

http://taylorandfrancis.metapress.com/(wttelrfhrigetqfq0sj5hc45)/app/home/contribution.asp?referrer=parent&backto=issue,8,12;journal,6,48;linkingpublicationresults,1:102430,1

 

2008:  The female reproductive track directly effect gender outcome

http://journals.cambridge.org/action/displayAbstract?fromPage=online&aid=1384548

 

  

Link to Lubricant pH: http://www.ingfertility.com/damage.html

Low pH favors girls. Douche help to lower pH.  Douches from the very best to the least best are:

-Vinegar 7% with calcium should be a pH of 4:

 http://www.in-gender.com/CS/forums/p/16792/145928.aspx#145928

 

1997-Why calcium is important in it: http://www.springerlink.com/content/3r7uxmkyxt18rcax/ -Lime douche (lime and water) should be a pH of 2.  This is low because lime pH tends to climb.-Store bought douche works the least but is the easiest.*Instead of douche you can use rephresh or replens: http://www.in-gender.com/cs/forums/t/46148.aspx

2006- Lowering men's pH of  semen.  Men who take Cranberry pills can lower their Ph.

http://ezinearticles.com/?Your-Semen---A--Diet--For-Better-Sperm-Taste&id=138648

DH’s  pH does matter! But his pH when coming out of the body should not drop below a 7 (below a 7 and he will be shotting dead sperm).  So you want his to be 7-7.5.  Once semen is out of the body it can be lowered to a pH of 4-4.5.

Several animal studies from 1950s confirm that male pH actually matters a lot! “Weir began in 1953 a new series of experiments in which he developed two strains of mice, one with an average blood pH of 7.42 and the other with an average blood pH of 7.46. He found that the interbreeding of those mice with the blood pH of 7.42 altered the female/male sex ratio from 50:50 to 60:40 and the interbreeding of the mice with the blood pH of 7.46 altered the ratio to about 40:60. By cross breeding or reciprocal mating of different sexes of the two strains, he demonstrated that it was the blood pH of the male and not the female which determined the sex ratio of the young.” WEIR, J. A. 1953. Association of blood pH with sex ratio in mice. J. of Heredity 44:133-138. WEIR, J. A. 1955. Male influence on the sex ratio of offspring in high and low blood pH. J. of Heredity 46:277-283. I found this at: http://www.freepatentsonline.com/4225405.html. (This link contains references to several other interesting studies, which might be worth looking into.) 

Oh wow thanks Tamara looks like I have some reading to do!

Great job M! I'm so glad you did this.