So, our experiment was awesome last night. Did everything in the order and could actually see the faster sperm in microscope. Worked well. I tested DH ph before we started anything and it was a 7.49, I tested the bottom half ( hopefully the XX's) after we left it in the incubator for an hour and pulled the eggwhite and fastest swimmers out of the top. The ph then was 8.22. What happen? heat? eggwhite?